HIV/AIDS-Related Cancer: Diagnosis

Approved by the Cancer.Net Editorial Board, 07/2017

ON THIS PAGE: You will find a list of common tests, procedures, and scans that doctors use to find the cause of a medical problem. Use the menu to see other pages.

Doctors use many tests to find, or diagnose, cancer. They also do tests to learn if cancer has spread to another part of the body from where it started. If this happens, it is called metastasis. For example, imaging tests can show if the cancer has spread. Imaging tests show pictures of the inside of the body. Doctors may also do tests to learn which treatments could work best.

For most types of cancer, a biopsy is the only sure way for the doctor to know whether an area of the body has cancer. In a biopsy, the doctor takes a small sample of tissue for testing in a laboratory. If a biopsy is not possible, the doctor may suggest other tests that will help make a diagnosis.

This list describes options for diagnosing an HIV/AIDS-related cancer. Not all tests listed below will be used for every person. Your doctor may consider these factors when choosing a diagnostic test:

  • The type of cancer suspected

  • Your signs and symptoms

  • Your age and medical condition

  • The results of earlier medical tests

The following tests may be used to diagnose an HIV/AIDS-related cancer or to determine if or where it has spread:

  • Biopsy. A biopsy is the removal of a small amount of tissue for examination under a microscope. Other tests can suggest that cancer is present, but only a biopsy can make a definite diagnosis. A pathologist then analyzes the sample(s). A pathologist is a doctor who specializes in interpreting laboratory tests and evaluating cells, tissues, and organs to diagnose disease.

  • Computed tomography (CT or CAT) scan. A CT scan creates a 3-dimensional picture of the inside of the body using x-rays taken from different angles. A computer combines these images into a detailed, cross-sectional view that shows any abnormalities or tumors. A CT scan can be used to measure the tumor’s size. Sometimes, a special dye called a contrast medium is given before the scan to provide better detail on the image. This dye is usually injected into a patient’s vein. CT scans of the chest and abdomen can help find cancer that has spread to the lungs, lymph nodes, or liver.

  • X-ray. An x-ray is a way to create a picture of the structures inside of the body, using a small amount of radiation.

  • Endoscopy. An endoscopy allows the doctor to see the inside the body with a thin, lighted, flexible tube called an endoscope. The person may be sedated as the tube is inserted through the mouth, down the esophagus, and into the stomach and small bowel. Sedation is giving medication to become more relaxed, calm, or sleepy. If abnormal areas are found, the doctor can remove a sample of tissue and check it for cancer. The doctor can examine the large intestine with a specific endoscopic procedure called a colonoscopy.

Kaposi sarcoma

  • Bronchoscopy. A bronchoscopy allows the doctor to see inside the lungs with a thin, lighted, flexible tube called a bronchoscope. The person is sedated as the tube is inserted through the mouth or nose, down through the windpipe, and into the breathing passages of the lungs. This procedure may be performed by a surgeon or a pulmonologist. A pulmonologist is a doctor who specializes in lung disease. Tiny tools inside the tube can collect samples of fluid and tissue, so the pathologist can examine the samples. Patients are given mild anesthesia during a bronchoscopy. Anesthesia is medication to block the awareness of pain.

  • Photography.Because many skin lesions can develop in different parts of the body, doctors regularly photograph parts of the skin. This is called mapping. It is done to find out whether new lesions have developed over time.

    • Positron emission tomography (PET) or PET-CT scan. A PET scan is usually combined with a CT scan (see above), called a PET-CT scan. However, you may hear your doctor refer to this procedure just as a PET scan. A PET scan is a way to create pictures of organs and tissues inside the body. A small amount of a radioactive sugar substance is injected into the patient’s body. This sugar substance is taken up by cells that use the most energy. Because cancer tends to use energy actively, it absorbs more of the radioactive substance. A scanner then detects this substance to produce images of the inside of the body. A nuclear medicine physician interprets the scan.

Non-Hodgkin lymphoma

A doctor can use this technique to look at both the structure and how much energy is used by the tumor and healthy tissues. If, after having 1 or more imaging tests, the doctor decides the lymphoma might be affecting the bone marrow, he or she may recommend a bone marrow biopsy.

  • Magnetic resonance imaging (MRI). An MRI uses magnetic fields, not x-rays, to produce detailed images of the body. MRI can also be used to measure the tumor’s size. A special dye called contrast medium is given before the scan to create a clearer picture. This dye can be injected into a patient’s vein or given as a pill to swallow. A radiologist interprets the scan. 

  • Bone marrow aspiration and biopsy. These 2 procedures are similar and typically done at the same time to examine the bone marrow. Bone marrow has both a solid and a liquid part. A bone marrow aspiration removes a sample of the fluid with a needle. A bone marrow biopsy is the removal of a small core of solid tissue using a needle.

    A pathologist or hematopathologist then analyzes the sample(s). A hematopathologist is a pathologist with specialized training in lymphoma, leukemia, and other blood cancers. A common site for a bone marrow aspiration and biopsy is the pelvic bone, which is located in the lower back by the hip. The skin in that area is numbed with medication beforehand. The patient may also receive other types of anesthesia to block the awareness of pain.

    Lymphoma often spreads to the bone marrow, so looking at a sample of the bone marrow can be important for diagnosing lymphoma and determining the stage. The doctor can also use the sample removed during the aspiration to find any chromosome changes.

  • Lumbar puncture, also called a “spinal tap.” Some aggressive lymphomas, especially Burkitt lymphoma, carry a risk of entering the spinal fluid surrounding the brain and spinal cord or going to the brain tissue itself.  As a result, a diagnostic lumbar puncture or brain imaging, usually an MRI, may be used in the initial staging. Chemotherapy may be used to treat or prevent central nervous system involvement.

  • Molecular testing of the tumor. Your doctor may recommend running laboratory tests on a tumor sample and/or bone marrow sample to identify specific genes, proteins, and other factors unique to the disease. Results of these tests will help decide whether your treatment options include a type of treatment called targeted therapy (see Treatment Options). There are different types of molecular and genetic testing:

    • Cytogenetics studies healthy and abnormal chromosomes in dividing cancer cells.

    • Fluorescent in situ hybridization (FISH) uses fluorescent probes under a special microscope to detect changes in specific chromosomes.

    • Flow cytometry looks at proteins that are on the surface or inside a cancer cell.

    • Polymerase chain reaction (PCR) detects specific DNA sequences that occur in some cancers.

    • Extended molecular profiling is used to broadly review tumor markers or to detect trace amounts of remaining cancer after treatment finishes. Although this is still being studied, it may become more commonly used in the near future.

Cervical cancer

  • Pap test. A Pap test is when the doctor gently scrapes the outside of the cervix and vagina, taking samples of the cells for testing.

    Improved Pap test methods have made it easier for doctors to find cancerous cells. Traditional Pap tests can be hard to read because cells can be dried out, covered with mucus or blood, or clump together on the slide.

  • The liquid-based cytology test (often referred to as ThinPrep or SurePath) transfers a thin layer of cells onto a slide after removing blood or mucus from the sample. Because the sample is preserved, other tests can be done at the same time.

  • Computer screening (often called AutoPap or Focal Point) uses a computer to scan the sample for abnormal cells.

  • Pelvic examination. In this examination, the doctor feels a woman’s uterus, vagina, ovaries, fallopian tubes, cervix, bladder, and rectum to check for any unusual changes. A Pap test is often done at the same time.

  • HPV typing. An HPV test is similar to a Pap test, where the test is done on a sample of cells from the patient’s cervix. The doctor may test for HPV at the same time as a Pap test or after Pap test results show abnormal changes to the cervix. Certain strains of HPV, such as HPV-16 and HPV-18, are seen more often in women with cervical cancer and may help confirm a diagnosis. If the doctor says the HPV test is “positive,” that means it found the presence of HPV. Many women have HPV but do not have cervical cancer, so HPV testing alone is not an accurate test for cervical cancer.

If the Pap test showed some abnormal cells and the HPV test is also positive, the doctor may suggest 1 or more of the following diagnostic tests: colposcopy, pelvic examination, x-ray, MRI, PET-CT scan, cystoscopy, proctoscopy, and/or laparoscopy. For details about diagnostic tests, visit the Cervical Cancer section.

After diagnostic tests are done, your doctor will review all of the results with you. If the diagnosis is cancer, these results also help the doctor describe the cancer. This is called staging.

The next section in this guide is Stages. It explains the system doctors use to describe the extent of an HIV/AIDS-related cancer. You may use the menu to choose a different section to read in this guide.